18S rDNA V4 sequences using HTS technology of individual LKYS01-SDC00... - SRA

SRX22052465: 18S rDNA V4 sequences using HTS technology of individual LKYS01-SDC001 N. scintillans cells
1 ILLUMINA (Illumina NovaSeq 6000) run: 132,839 spots, 66.4M bases, 22.2Mb downloads

Design: Sequencing libraries were generated using NEBNext DNA Library Prep Kit. The genomic DNA was randomly fragmented to a size of 350bp by shearing; DNA fragments were end polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing, and further PCR enriched by P5 and indexed P7 oligos. The PCR products were purified (AMPure XP system, United States), followed by analysis for size distribution. Qualified libraries were sequenced on an Illumina platform. The sequencing strategy was Novaseq PE200 (Illumina, San Diego, USA).

Submitted by: Institute of Oceanology, Chinese Academy of Sciences

Study: Metabarcoding analysis of 18S rDNA V4 sequenced obtained in single Noctiluca scintillans cells

PRJNA1026735 • SRP465534 • All experiments • All runs

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To evaluate the diversity of 18S rDNA V4 in N. scintillans, distinguishing between intra-genomic molecular marker sequences variations and intra-species genetic diversity.

Sample:

SAMN37756287 • SRS19123067 • All experiments • All runs

Organism: Noctiluca scintillans

Library:

Name: LKYS01-SDC001

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 132,839 spots, 66.4M bases, 22.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR26344760	132,839	66.4M	22.2Mb	2023-10-11

ID:: 29976647

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